The goal of the Sound Life Project is to understand the diversity of the immune system outside the context of disease, and how people with different immune systems that still fall within the “normal” range might respond differently to things like infection or vaccination.
Conducted in collaboration with Dr. Jane Buckner, the Sound Life Project is a study involving 100 adults with no known chronic diseases who came in for 10 study visits over two years. They were enrolled in two cohorts based on their age, one cohort made up of people 25-35 years old and a second cohort made up of people 55-65 years old.
Sound Life Project Participants completed extensive surveys about their health history and lifestyle, submitted samples for standard clinical testing and deep immunological profiling at every visit, and completed weekly surveys updating us about their travel history and health. These extensive data have allowed us to ask interesting questions about healthy immune systems.
For example, we and others showed that healthy adult immune systems maintain stable baseline frequencies of many populations of circulating immune cells. We characterized this immune baseline for each Sound Life Project participant. Then we looked at moments when the participant’s immune system was challenged either in vivo by a vaccination or in vitro using stimulation assays. We characterized the response to these immune perturbations using cytometric and genomic techniques. We are now applying novel analytical methods to identify features of the immune baseline that can help define the heterogeneous response to perturbation.
Featured Publications
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Jun 2025
Longitudinally stable T cell function and innate immune activation distinguish healthy adult immunotypes.
Sci Transl MedSmithmyer ME, Hu A, Dufort MJ, Hocking AM, Wiedeman AE, Fasano KJ, Torgerson TR, Skene PJ, Reading J, Li X, Gong Q, Long SA, Lacy-Hulbert A, Gustafson CE, Buckner JH, Speake C, members of the BRI Sound Life Project Team, BRI Sound Life Project Team -
Oct 2024
Longitudinal Multi-omic Immune Profiling Reveals Age-Related Immune Cell Dynamics in Healthy Adults.
bioRxivGong Q, Sharma M, Kuan EL, Glass MC, Chander A, Singh M, Graybuck LT, Thomson ZJ, LaFrance CM, Zaim SR, Peng T, Okada LY, Genge PC, Henderson KE, Dornisch EM, Layton ED, Wittig PJ, Heubeck AT, Mukuka NM, Reading J, Roll CR, Hernandez V, Parthasarathy V, Stuckey TJ, Musgrove B, Swanson E, Lord C, Weiss MDA, Phalen CG, Mettey RR, Lee KJ, Johanneson JB, Kawelo EK, Garber J, Krishnan U, Smithmyer M, Wherry EJ, Vella L, Henrickson SE, Kopp MS, Savage AK, Becker LA, Meijer P, Coffey EM, Goronzy JJ, Speake C, Bumol TF, Goldrath AW, Torgerson TR, Li XJ, Skene PJ, Buckner JH, Gustafson CE -
Apr 2022
A simple strategy for sample annotation error detection in cytometry datasets.
Cytometry ASmithmyer ME, Wiedeman AE, Skibinski DAG, Savage AK, Acosta-Vega C, Scheiding S, Gersuk VH, O'Rourke C, Long SA, Buckner JH, Speake C
Additional Research Projects

Rigor and Reproducibility in T1D Biomarker Research
